Journal: Inflammation

Article Title: MAGL Inhibition Relieves Psoriasiform Inflammation and Pruritus Via Modulation of ALOX12-12-HETE Axis in Mice

doi: 10.1007/s10753-025-02383-5

Figure Lengend Snippet: Anti-inflammatory and antipruritic effects of JZL184 are partially mediated by CB2R. ( A ) The flowchart of the AM251, AM630, and JZL184 treatment in IMQ-induced psoriasis model. ( B ) Representative photographs of the dorsal skin and ( C ) H&E staining images of skin lesions on Day 5. ( D ) Epidermal thickness quantified from five randomly selected fields per section for each mouse ( n = 5 mice per group). ( E ) Comparative analysis of PASI scores across groups over the experimental period. The PASI scores were calculated daily from the dorsal neck skin ( n = 5 per group). ( F - G ) Representative photographs of the spleens. Spleen index (mg/g) = spleen weight (mg) ÷ mouse body weight (g) ( n = 5 per group). (H) Scratching bouts recorded over 1 h, measured every other day ( n = 6 per group). Data shown in the graph are presented as mean ± SEM. The P values were calculated by one-way ANOVA and Tukey’stest ( D , G ) or two-way ANOVA and Holm-Šídák test (E, H). * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, not significant when compared. Scale bar = 100 μm

Article Snippet: CB2R knockout male mice (strain: B6.129P2-Cnr2 tm1Dgen/J ) of the same age and weight were generated by Jackson Laboratories using ES cell-targeted homologous recombination, resulting in a permanent, global deletion of the Cnr2 gene.

Techniques: Staining

Journal: Inflammation

Article Title: MAGL Inhibition Relieves Psoriasiform Inflammation and Pruritus Via Modulation of ALOX12-12-HETE Axis in Mice

doi: 10.1007/s10753-025-02383-5

Figure Lengend Snippet: Anti-inflammatory and antipruritic effects of JZL184 in psoriasis are reversed in CB2R−/− mice. ( A - C ) Representative photographs of the dorsal skin and H&E staining images of the skin lesions on Day 5. The epidermal thickness was calculated from five randomly selected fields per section for each mouse ( n = 5 mice per group). ( D , E ) Representative images of the spleens. Spleen index = spleen weight (mg) ÷ mouse body weight (g) ( n = 5 per group). ( F ) Comparative analysis of PASI scores across groups over the full course of the experiment. The PASI scores were assessed daily ( n = 5 per group). ( G ) Scratch bouts in an hour were counted every other day ( n = 5 per group). Data shown in the graph are presented as mean ± SEM. The P values were calculated by one-way ANOVA and Tukey’stest ( C , E ) or two-way ANOVA and Holm-Šídák test ( F , G ). * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, not significant when compared. Scale bar = 100 μm

Article Snippet: CB2R knockout male mice (strain: B6.129P2-Cnr2 tm1Dgen/J ) of the same age and weight were generated by Jackson Laboratories using ES cell-targeted homologous recombination, resulting in a permanent, global deletion of the Cnr2 gene.

Techniques: Staining

Journal: Inflammation

Article Title: MAGL Inhibition Relieves Psoriasiform Inflammation and Pruritus Via Modulation of ALOX12-12-HETE Axis in Mice

doi: 10.1007/s10753-025-02383-5

Figure Lengend Snippet: JZL184 mediates psoriasis-like dermatitis in mice via modulating arachidonic acid metabolism. ( A ) Concentrations of AA in skin lesions detected by UHPLC-MS/MS ( n = 4 per group). ( B ) Heatmap of 50 identified AA metabolites from skin tissues collected 5 days after IMQ treatment. Different samples (shown as columns) with increased or decreased levels of metabolites (shown as rows) are indicated by red or blue. Metabolites are classified into three categories based on the main metabolizing enzymes ( n = 4 per group). ( C - F ) Western blot analysis of ALOX5, ALOX12 and ALOX15 protein in skin tissues, with GAPDH and ACTB as loading controls ( n = 5 per group). ( G ) Representative immunofluorescence images of CB2R + (green) and ALOX12 + (red) cell in skin tissues. The arrows indicated CB2R and ALOX12 double-positive cells. Data shown in the graph are presented as mean ± SEM. The P values were calculated by one-way ANOVA and Tukey’stest ( A , E , F ). * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, not significant when compared. Scale bar = 50 μm

Article Snippet: CB2R knockout male mice (strain: B6.129P2-Cnr2 tm1Dgen/J ) of the same age and weight were generated by Jackson Laboratories using ES cell-targeted homologous recombination, resulting in a permanent, global deletion of the Cnr2 gene.

Techniques: Tandem Mass Spectroscopy, Western Blot, Immunofluorescence